Characterization of Bioaugmented Isolates for Domestic Wastewater Treatment and The Potential Utilization for Plants

Abstract

Earth without water is impossible. Sustainable water is something that must be done by humankind. Water pollution in big cities in Indonesia tends to be dominated by domestic wastewater. One of the ways to maintain water sustainability that is more environmentally friendly is by bioaugmentation in domestic wastewater. This study aims to determine the characteristics of selected isolates used in domestic wastewater bioaugmentation. The isolates came from 3 isolates from washing rice wastewater, and 1 isolate from tape yeast. The four isolates were characterized using molecular approaches and morphological tests. The test of the isolates' ability to degrade starch, protein, oil and detergents in domestic wastewater was carried out to see the potential of the isolates as bioaugmentation agents. Isolates were identified using the 16S rRNA gene for bacterial isolates and ITS genes for yeast isolates. Isolates UMJ-A1, UMJ-R, UMJ-A2 and UMJ-L respectively had a high similarity with Burkholderia ambifaria, Burkholderia sp. Trichosporon asahii and Pichia kudriavzevii based on the results of their molecular and morphological identification. Additional isolates were obtained from InaCC LIPI with the identity of Pseudomonas putida isolates InaCCB72. The UMJ-A1 isolate was suspected of being a pathogen, so it was not included in the next test. The characteristics of the degradation ability of the mixture of the four remaining microbes as bioaugmentation agents in domestic wastewater have the potential to reduce pollution, as well as produce growth regulators that can be used for plants.